Proteintech, 15502-1-AP, transgelin/SM22-specific Polyclonal antibody

Size: 20ul / 150ul The transgelin/SM22-specific (15502-1-AP) by Proteintech is a Polyclonal antibody targeting transgelin/SM22-specific in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse colon tissue Positive IHC detected in: human heart tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:300-1:600 Immunohistochemistry (IHC): IHC : 1:100-1:4000 Background Information The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transgelin Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22-25 kDa GenBank Accession Number: NM_003186 Gene Symbol: transgelin/SM22 Gene ID (NCBI): 6876 RRID: AB_2199361 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01995 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.