Proteintech, 15513-1-AP, Cardiac Troponin T Polyclonal antibody

Size: 20ul / 150ul The Cardiac Troponin T (15513-1-AP) by Proteintech is a Polyclonal antibody targeting Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse, rat samples 15513-1-AP targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: pig heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Positive IF-Fro detected in: mouse heart tissue Positive IF/ICC detected in: AC16 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK Predict reactive species Full Name: troponin T type 2 (cardiac) Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 34-40 kDa GenBank Accession Number: BC002653 Gene Symbol: Cardiac Troponin T Gene ID (NCBI): 7139 RRID: AB_2206563 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P45379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.