Proteintech, 15518-1-AP, Beta Galactosidase Polyclonal antibody

Size: 20ul / 150ul The Beta Galactosidase (15518-1-AP) by Proteintech is a Polyclonal antibody targeting Beta Galactosidase in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse samples 15518-1-AP targets Beta Galactosidase in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SH-SY5Y cells Positive IHC detected in: human prostate cancer tissue, human gliomas tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue, human prostate cancer tissue, mouse liver tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information What is the molecular weight of β-galactosidase (GLB1)?The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lowermolecular weight values.What is the function of GLB1?The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzingthe hydrolysisoflactoseto monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymericD-galactosylresidues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).How is GLB1 used in our food?GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturingof lactose-hydrolyzed products (PMID: 28500401).How is GLB1 part of a positive feedback loop?GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon andbinds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).What organisms express GLB1?GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals.Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values(PMID: 28500401).How is GLB1 utilized in transgenic animals?Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. ThelacZgene ofE. colihas been used to identifycis-acting DNA elements that play a key role for the regulation ofgene expressionin mice. ThelacZgene can be incorporated into an endogenous gene, and it is frequently a used to indicatethe success of Cre-loxP recombination events, which allows temporal control oflacZexpression.Mice apart of the Rosa26R line that also express Cre ubiquitously express Cre-induciblelacZ(PMID: 26394634). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7792 Product name: Recombinant human Beta galactosidase protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 329-677 aa of BC007493 Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV Predict reactive species Full Name: galactosidase, beta 1 Calculated Molecular Weight: 76 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC007493 Gene Symbol: GLB1/Beta-galactosidase Gene ID (NCBI): 2720 RRID: AB_2263448 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16278 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.