Proteintech, 15526-1-AP, PAFAH Polyclonal antibody

Size: 20ul / 150ul The PAFAH (15526-1-AP) by Proteintech is a Polyclonal antibody targeting PAFAH in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 15526-1-AP targets PAFAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells, mouse spleen tissue, mouse liver tissue, RAW264.7 Positive IHC detected in: human tonsillitis tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7227 Product name: Recombinant human PLA2G7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 92-438 aa of BC038452 Sequence: RLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIE Predict reactive species Full Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 43-67 kDa GenBank Accession Number: BC038452 Gene Symbol: PLA2G7 Gene ID (NCBI): 7941 RRID: AB_2878146 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13093 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.