Proteintech, 15542-1-AP, MRPL49 Polyclonal antibody

Size: 20ul / 150ul The MRPL49 (15542-1-AP) by Proteintech is a Polyclonal antibody targeting MRPL49 in WB, IHC, IP, ELISA applications with reactivity to human samples 15542-1-AP targets MRPL49 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, A549 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7898 Product name: Recombinant human MRPL49 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC004378 Sequence: MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF Predict reactive species Full Name: mitochondrial ribosomal protein L49 Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 19 kDa GenBank Accession Number: BC004378 Gene Symbol: MRPL49 Gene ID (NCBI): 740 RRID: AB_2146189 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13405 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.