Proteintech, 15558-1-AP, ZNF259 Polyclonal antibody

Size: 20ul / 150ul The ZNF259 (15558-1-AP) by Proteintech is a Polyclonal antibody targeting ZNF259 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 15558-1-AP targets ZNF259 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human brain tissue, DU 145 cells, HeLa cells, HepG2 cells Positive IHC detected in: human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ZNF259, also named as Zpr1, is an evolutionarily conserved protein characterized by two C4 zinc fingers and two Zpr1-specific conserved homology domains. Zpr1 was first identified in mammalian cells as a cytoplasmic protein that was capable of binding the intracellular domain of the unactivated EGF receptor (EGFR). The MW of this protein is 51 kDa, and this antibody specially recognises the 51 kDa protein. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7980 Product name: Recombinant human ZNF259 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 111-459 aa of BC004256 Sequence: EDMNREVVKTDSAATRIPELDFEIPAFSQKGALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQVASPFTLIIDDPSGNSFVENPHAPQKDDALVITHYNRTRQQEEMLGLQEEAPAEKPEEEDLRNEVLQFSTNCPECNAPAQTNMKLVQIPHFKEVIIMATNCENCGHRTNEVKSGGAVEPLGTRITLHITDASDMTRDLLKSETCSVEIPELEFELGMAVLGGKFTTLEGLLKDIRELVTKNPFTLGDSSNPGQTERLQEFSQKMDQIIEGNMKAHFIMDDPAGNSYLQNVYAPEDDPEMKVERYKRTFDQNEELGLNDMKTEGYEAGLAPQR Predict reactive species Full Name: zinc finger protein 259 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC004256 Gene Symbol: ZNF259 Gene ID (NCBI): 8882 RRID: AB_10858477 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75312 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.