Proteintech, 15594-1-AP, CDCA3 Polyclonal antibody

Size: 20ul / 150ul The CDCA3 (15594-1-AP) by Proteintech is a Polyclonal antibody targeting CDCA3 in IHC, IF/ICC, ELISA applications with reactivity to human samples 15594-1-AP targets CDCA3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CDCA3, also named C8, GRCC8, and TOME1, is an F-box-like protein required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at the G2/M phase. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7951 Product name: Recombinant human CDCA3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-268 aa of BC002551 Sequence: MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETEDSKSNLPPEPVLPPEAPLSSELDLPLGTQLSVEEQMPPWNQTEFPSKQVFSKEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQGQDHDKENQHFPLVES Predict reactive species Full Name: cell division cycle associated 3 Calculated Molecular Weight: 29 kDa GenBank Accession Number: BC002551 Gene Symbol: CDCA3 Gene ID (NCBI): 83461 RRID: AB_2878154 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99618 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.