Proteintech, 15597-1-AP, MCM3 Polyclonal antibody

Size: 20ul / 150ul The MCM3 (15597-1-AP) by Proteintech is a Polyclonal antibody targeting MCM3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 15597-1-AP targets MCM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, Raji cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7966 Product name: Recombinant human MCM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 201-503 aa of BC001626 Sequence: IQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLR Predict reactive species Full Name: minichromosome maintenance complex component 3 Calculated Molecular Weight: 91 kDa Observed Molecular Weight: 102-105 kDa GenBank Accession Number: BC001626 Gene Symbol: MCM3 Gene ID (NCBI): 4172 RRID: AB_2141973 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25205 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.