Proteintech, 15709-1-AP, UPK3B Polyclonal antibody

Size: 20ul / 150ul The UPK3B (15709-1-AP) by Proteintech is a Polyclonal antibody targeting UPK3B in IP, IHC, ELISA applications with reactivity to human, mouse samples 15709-1-AP targets UPK3B in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: mouse testis tissue Positive IHC detected in: human bladder tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-3b (UPK3B), also named as p35, is a single-pass type I membrane protein. This protein dimerizes with uroplakin-1b (UPK1B) to exit out of endoplasmic reticulum, which is is an important early step in urothelial plaque assembly (PMID: 12446744). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8326 Product name: Recombinant human UPK3B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC004304 Sequence: MGLPWGQPHLGLQMLLLALNCLRPSLSLGEWGSWMDASSQTQGAGGPAGVIGPWAPAPLRLGEAAPGTPTPVSVAHLLSPVATELVPYTPQITAWDLEGKVTATTFSLEQPRCVFDGLASASDTVWLVVAFSNASRGFQNPETLADIPASPQLLTDGHYMTLPLSPDQLPCGDPMAGSGGAPVLRVGHDHGCHQQPFCNAPLPGPGPYREDPRIHRHLARAAKWQHDRHYLHPLFSGRPPTLGLLGSLYHALLQPVVAGGGPGAAADRLLHGQALHDPPHPTQRGRHTAGGLQAWPGPPPQPQPLAWPLCMGLGEMGRRE Predict reactive species Full Name: uroplakin 3B Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC004304 Gene Symbol: UPK3B Gene ID (NCBI): 80761 RRID: AB_2878170 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BT76 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.