Proteintech, 15930-1-AP, ART3 Polyclonal antibody

Size: 20ul / 150ul The ART3 (15930-1-AP) by Proteintech is a Polyclonal antibody targeting ART3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 15930-1-AP targets ART3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human testis tissue, rat testis tissue, mouse testis Positive IHC detected in: mouse testis tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ART3 is a GPI-anchored mono-ADP-ribosyltransferase enriched on the sperm cell surface, where it participates in spermatid maturation and membrane remodeling. Localized to lipid rafts, ART3 likely modulates cell-cell signaling and extracellular protein modification. Its upregulation in multiple cancers suggests additional roles in migration, invasion, and tumor aggressiveness. Despite incomplete characterization of its enzymatic activity, ART3 remains a key regulator at the interface of reproductive biology, membrane signaling, and pathology. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8775 Product name: Recombinant human ART3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-364 aa of BC008397 Sequence: EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSASLG Predict reactive species Full Name: ADP-ribosyltransferase 3 Calculated Molecular Weight: 389 aa, 44 kDa Observed Molecular Weight: 38-47 kDa GenBank Accession Number: BC008397 Gene Symbol: ART3 Gene ID (NCBI): 419 RRID: AB_2060870 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13508 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.