Proteintech, 16033-1-AP, SRP19 Polyclonal antibody

Size: 20ul / 150ul The SRP19 (16033-1-AP) by Proteintech is a Polyclonal antibody targeting SRP19 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16033-1-AP targets SRP19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human liver tissue, A549 cells, HeLa cells, K-562 cells, mouse kidney tissue, mouse liver tissue, mouse ovary tissue, Raji cells Positive IP detected in: mouse kidney tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA. Binding of SRP19 leads to restructuring of both helix 6 and 8, causing local changes at the SRP54-binding site. This antibody is a rabbit polyclonal antibody raised against full length SRP19 of human origin. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8903 Product name: Recombinant human SRP19 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC010947 Sequence: MACTAARSPADQDRFICIYPAYLNNKKTIAEGRRIPISKAVENPTATEIQDVCSAVGLNVFLEKNKMYSREWNRDVQYRGRVRVQLKQEDGSLCLVQFPSRKSVMLYAAEMIPKLKTRTQKTGGADQSLQQGEGSKKGKGKKKK Predict reactive species Full Name: signal recognition particle 19kDa Calculated Molecular Weight: 144 aa, 16 kDa Observed Molecular Weight: 18-25 kDa GenBank Accession Number: BC010947 Gene Symbol: SRP19 Gene ID (NCBI): 6728 RRID: AB_11041714 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09132 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.