Proteintech, 16064-1-AP, PMPCB Polyclonal antibody

Size: 20ul / 150ul The PMPCB (16064-1-AP) by Proteintech is a Polyclonal antibody targeting PMPCB in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16064-1-AP targets PMPCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue Positive IP detected in: MCF-7 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8937 Product name: Recombinant human PMPCB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC010398 Sequence: MAAAAARVVLSSAARRRLWGFSESLLIRGAAGRSLYFGENRLRSTQAATQVVLNVPETRVTCLESGLRVASEDSGLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVSHDELLDLAKFHFGDSLCTHKGEIPALPPCKFTGSEIRVRDDKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHS Predict reactive species Full Name: peptidase (mitochondrial processing) beta Calculated Molecular Weight: 489 aa, 54 kDa Observed Molecular Weight: 43-52 kDa GenBank Accession Number: BC010398 Gene Symbol: PMPCB Gene ID (NCBI): 9512 RRID: AB_2167122 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75439 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.