Proteintech, 16150-1-AP, BAG1S/1M/1L Polyclonal antibody

Size: 20ul / 150ul The BAG1S/1M/1L (16150-1-AP) by Proteintech is a Polyclonal antibody targeting BAG1S/1M/1L in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 16150-1-AP targets BAG1S/1M/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, Jurkat cells, mouse liver tissue Positive IP detected in: Jurkat cells Positive IHC detected in: human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: BCL2-associated athanogene Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 46-50 kDa, 41-46 kDa, 25-33 kDa GenBank Accession Number: NM_004323 Gene Symbol: BAG1 Gene ID (NCBI): 573 RRID: AB_2062288 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99933 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.