Proteintech, 16152-1-AP, HDAC2-specific Polyclonal antibody

Size: 20ul / 150ul The HDAC2-specific (16152-1-AP) by Proteintech is a Polyclonal antibody targeting HDAC2-specific in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 16152-1-AP targets HDAC2-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A431 cells, HeLa cells, human liver tissue Positive IP detected in: A431 cells Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone deacetylase 2 Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa, 55 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: NM_001527 Gene Symbol: HDAC2 Gene ID (NCBI): 3066 RRID: AB_2118514 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.