Proteintech, 16174-1-AP, ICAM-1/CD54 Polyclonal antibody

Size: 20ul / 150ul The ICAM-1/CD54 (16174-1-AP) by Proteintech is a Polyclonal antibody targeting ICAM-1/CD54 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to mouse samples 16174-1-AP targets ICAM-1/CD54 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse lung tissue, mouse spleen tissue Positive IP detected in: mouse spleen tissue Positive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse lung tissue Positive IF/ICC detected in: 4T1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Where is ICAM-1 expressed?Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.What is the function of ICAM-1?ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.What is the role of ICAM-1 in disease?Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades.Free Radic. Biol. Med.28,1379-86 (2000).Long, E. O. ICAM-1: getting a grip on leukocyte adhesion.J. Immunol.186,5021-3 (2011).Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues.Curr. Opin. Hematol.22,53-59 (2015).Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating.J. Virol.77,6101-7 (2003). Specification Tested Reactivity: mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: intercellular adhesion molecule 1 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 90-110 kDa GenBank Accession Number: NM_010493 Gene Symbol: ICAM-1 Gene ID (NCBI): 15894 RRID: AB_2248702 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13597 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.