Proteintech, 16202-1-AP, PLEKHM1 Polyclonal antibody

Size: 20ul / 150ul The PLEKHM1 (16202-1-AP) by Proteintech is a Polyclonal antibody targeting PLEKHM1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: ROS1728 cells, mouse liver tissue Positive IHC detected in: mouse kidney tissue, human liver cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PLEKHM1, also known as AP162, OPTB6, belongs to the Pleckstrin homology domain protein family. PLEKHM1 functions as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events within the endolysosomal system, thereby coupling autophagic and endocytic trafficking pathways. In osteoclasts, PLEKHM1 is crucial for bone resorption by regulating the vesicle transport process and ensuring the formation of the ruffled border. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9145 Product name: Recombinant human PLEKHM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 708-1056 aa of BC064361 Sequence: EALKCFRIRNNEKMLSDSHGVETIRDILPDTSLGGPSFFKIITAKAVLKLQAGNAEEAALWRDLVRKVLASYLETAEEAVTLGGSLDENCQEVLKFATRENGFLLQYLVAIPMEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDICHQDDASVIPARIIHNWDLTKRPICRQALKFLTQIRAQPLINLQMVNASLYEHVERMHLIGRRREQLKLLGDYLGLCRSGALKELSKRLNHRNYLLESPHRFSVADLQQIADGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICQHHDIIFPFEFDTTVRCAECKTVFHQSCQAVVKKGCPRCARRRKYQEQNIFA Predict reactive species Full Name: pleckstrin homology domain containing, family M (with RUN domain) member 1 Calculated Molecular Weight: 117 kDa Observed Molecular Weight: 117 kDa GenBank Accession Number: BC064361 Gene Symbol: PLEKHM1 Gene ID (NCBI): 9842 RRID: AB_2878228 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4G2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.