Proteintech, 16207-1-AP, ALG14 Polyclonal antibody

Size: 20ul / 150ul The ALG14 (16207-1-AP) by Proteintech is a Polyclonal antibody targeting ALG14 in IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 16207-1-AP targets ALG14 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ALG14 (UDP-N-Acetylglucosaminyltransferase Subunit ALG14 Homolog) is a protein involved in the process of N-linked glycosylation, which is essential for protein folding and stability. ALG14 forms a heterodimeric UDP-N-acetylglucosamine transferase (GnTase) with ALG13, which catalyzes a key step in the synthesis of the lipid-linked oligosaccharide (LLO) precursor during N-linked glycosylation (PMID: 36200043). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9178 Product name: Recombinant human ALG14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-216 aa of BC011706 Sequence: RSMDVTPRESLSILVVAGSGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMYTKYYIHRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLLGILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSVYLGRIV Predict reactive species Full Name: asparagine-linked glycosylation 14 homolog (S. cerevisiae) Calculated Molecular Weight: 216 aa, 24 kDa GenBank Accession Number: BC011706 Gene Symbol: ALG14 Gene ID (NCBI): 199857 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96F25 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.