Proteintech, 16219-1-AP, VPS39 Polyclonal antibody

Size: 20ul / 150ul The VPS39 (16219-1-AP) by Proteintech is a Polyclonal antibody targeting VPS39 in IP, IHC, ELISA applications with reactivity to human, mouse samples 16219-1-AP targets VPS39 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: mouse brain tissue Positive IHC detected in: human heart tissue, human small intestine tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9267 Product name: Recombinant human VPS39 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 437-786 aa of BC015817 Sequence: ERTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFKGLAIPYLEHIIHVWEETGSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGELGEYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDTRMAEEYCHKHYDRNKDGNKDVYLSLLRMYLSPPSIHCLGPIKLELLEPKANLQAALQVLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLRVQEERILHQQVKCIITEEKVCMVCKKKIGNSAFARYPNGVVVHYFCSKEVNPADT Predict reactive species Full Name: vacuolar protein sorting 39 homolog (S. cerevisiae) Calculated Molecular Weight: 886 aa, 102 kDa Observed Molecular Weight: 102 kDa GenBank Accession Number: BC015817 Gene Symbol: VPS39 Gene ID (NCBI): 23339 RRID: AB_11043179 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96JC1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.