Proteintech, 16253-1-AP, EXO1/Exonuclease 1 Polyclonal antibody

Size: 20ul / 150ul The EXO1/Exonuclease 1 (16253-1-AP) by Proteintech is a Polyclonal antibody targeting EXO1/Exonuclease 1 in WB, IP, ELISA applications with reactivity to human, mouse samples 16253-1-AP targets EXO1/Exonuclease 1 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells, Raji cells Positive IP detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9253 Product name: Recombinant human EXO1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 496-846 aa of BC007491 Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ Predict reactive species Full Name: exonuclease 1 Calculated Molecular Weight: 94 kDa Observed Molecular Weight: 115-120 kDa GenBank Accession Number: BC007491 Gene Symbol: EXO1 Gene ID (NCBI): 9156 RRID: AB_2278140 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UQ84 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.