Proteintech, 16354-1-AP, BLZF1 Polyclonal antibody

Size: 20ul / 150ul The BLZF1 (16354-1-AP) by Proteintech is a Polyclonal antibody targeting BLZF1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 16354-1-AP targets BLZF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SCaBER cells, Jurkat cells, K-562 cells Positive IHC detected in: human liver cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9564 Product name: Recombinant human BLZF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 48-400 aa of BC020716 Sequence: QSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL Predict reactive species Full Name: basic leucine zipper nuclear factor 1 Calculated Molecular Weight: 400 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC020716 Gene Symbol: BLZF1 Gene ID (NCBI): 8548 RRID: AB_2918045 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H2G9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.