Proteintech, 16436-1-AP, FARS2 Polyclonal antibody

Size: 20ul / 150ul The FARS2 (16436-1-AP) by Proteintech is a Polyclonal antibody targeting FARS2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 16436-1-AP targets FARS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information FARS2, a nuclear gene located on chromosome 6 (6p25.1), encodes the mitochondrial phenylalanyl-tRNA synthetase (mtPheRS), which transfers phenylalanine (Phe) to its cognate tRNA in mitochondria (PMID: 32774346). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9750 Product name: Recombinant human FARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 109-451 aa of BC021112 Sequence: LFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGVEGRF Predict reactive species Full Name: phenylalanyl-tRNA synthetase 2, mitochondrial Calculated Molecular Weight: 451 aa, 52 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC021112 Gene Symbol: FARS2 Gene ID (NCBI): 10667 RRID: AB_2102499 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95363 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.