Proteintech, 16479-1-AP, ADO Polyclonal antibody

Size: 20ul / 150ul The ADO (16479-1-AP) by Proteintech is a Polyclonal antibody targeting ADO in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: fetal human brain tissue, human brain tissue, mouse spleen tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9598 Product name: Recombinant human ADO protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-270 aa of BC018660 Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP Predict reactive species Full Name: 2-aminoethanethiol (cysteamine) dioxygenase Calculated Molecular Weight: 270 aa, 30 kDa Observed Molecular Weight: 28 kDa-30 kDa GenBank Accession Number: BC018660 Gene Symbol: ADO Gene ID (NCBI): 84890 RRID: AB_2222204 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96SZ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.