Proteintech, 16521-1-AP, PMEPA1 Polyclonal antibody

Size: 20ul / 150ul The PMEPA1 (16521-1-AP) by Proteintech is a Polyclonal antibody targeting PMEPA1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16521-1-AP targets PMEPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: L02 cells, U2OS cells Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information PMEPA1, also known as TMEPAI, was first discovered as androgen inducible gene in hormone responsive LNCaP cells. PMEPA1 is highly expressed in various solid tumors and is known to play important roles in the transforming growth factor-β(TGF-β) signaling pathway (PMID: 27697531). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9744 Product name: Recombinant human PMEPA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 57-280 aa of BC015918 Sequence: HYKLSARSFISRHSQGRRREDALSSEGCLWPSESTVSGNGIPEPQVYAPPRPTDRLAVPPFAQRERFHRFQPTYPYLQHEIDLPPTISLSDGEEPPPYQGPCTLQLRDPEQQLELNRESVRAPPNRTIFDSDLMDSARLGGPCPPSSNSGISATCYGSGGRMEGPPPTYSEVIGHYPGSSFQHQQSSGPPSLLEGTRLHHTHIAPLESAAIWSKEKDKQKGHPL Predict reactive species Full Name: prostate transmembrane protein, androgen induced 1 Calculated Molecular Weight: 287 aa, 32 kDa Observed Molecular Weight: 32-36 kDa GenBank Accession Number: BC015918 Gene Symbol: PMEPA1 Gene ID (NCBI): 56937 RRID: AB_2284052 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969W9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.