Proteintech, 16699-1-AP, NKD2 Polyclonal antibody

Size: 20ul / 150ul The NKD2 (16699-1-AP) by Proteintech is a Polyclonal antibody targeting NKD2 in WB, ELISA applications with reactivity to human, mouse, rat samples 16699-1-AP targets NKD2 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, HEK-293 cells, mouse colon tissue Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Background Information Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10094 Product name: Recombinant human Naked2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-311 aa of BC012176 Sequence: MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSQLCEKRSSAPRTHSGDKARGVGLCRELWSQAGHPQWPGPFPSGLVAV Predict reactive species Full Name: naked cuticle homolog 2 (Drosophila) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC012176 Gene Symbol: NKD2 Gene ID (NCBI): 85409 RRID: AB_2151468 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969F2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.