Proteintech, 16702-1-AP, CYP20A1 Polyclonal antibody

Size: 20ul / 150ul The CYP20A1 (16702-1-AP) by Proteintech is a Polyclonal antibody targeting CYP20A1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10099 Product name: Recombinant human CYP20A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 112-462 aa of BC020616 Sequence: SGGGSVSENHMREKLYENGVTDSLKSNFALLLKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGFLDGSLDKNMTRKKQYEDALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLASCIITAKLCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLRYCQHVLCETVRTAKLTPVSAQFQDIEGKIDRFIIPRETLVLYALGVVLQDPNTWPSPHKFDPDRFDDELVMKTFSSLGFSGTQECPELRFAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY Predict reactive species Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1 Calculated Molecular Weight: 462 aa, 52 kDa Observed Molecular Weight: 45-52 kDa GenBank Accession Number: BC020616 Gene Symbol: CYP20A1 Gene ID (NCBI): 57404 RRID: AB_2261395 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UW02 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.