Proteintech, 16744-1-AP, IL-15RA Polyclonal antibody

Size: 20ul / 150ul The IL-15RA (16744-1-AP) by Proteintech is a Polyclonal antibody targeting IL-15RA in WB, IHC, ELISA applications with reactivity to human samples 16744-1-AP targets IL-15RA in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, K-562 cells, Ramos cells Positive IHC detected in: human liver cancer tissue, human spleen tissue, human kidney tissue, human heart tissue, human testis tissue, human skin tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights have been reported (PMID: 10480910; 15976182; 15671076; 17912462). Specification Tested Reactivity: human Cited Reactivity: human, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10151 Product name: Recombinant human IL-15RA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC107777 Sequence: MSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKTWELTASASHQPPGVYPQGHSDTTVAISTSTVLLCGLSAVSLLACYLKSRQTPPLASVEMEAMEALPVTWGTSSRDEDLENCSHHL Predict reactive species Full Name: interleukin 15 receptor, alpha Calculated Molecular Weight: 231 aa, 24 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC107777 Gene Symbol: IL-15RA Gene ID (NCBI): 3601 RRID: AB_2233644 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13261 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.