Proteintech, 16755-1-AP, RCC2 Polyclonal antibody

Size: 20ul / 150ul The RCC2 (16755-1-AP) by Proteintech is a Polyclonal antibody targeting RCC2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16755-1-AP targets RCC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, PC-3 cells, HeLa cells, HUVEC cells, MOLT-4 cells, NCI-H1299 cells Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse testis tissue, rat colon tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10271 Product name: Recombinant human RCC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 168-522 aa of BC042141 Sequence: EGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACGRNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGCPEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL Predict reactive species Full Name: regulator of chromosome condensation 2 Calculated Molecular Weight: 522 aa, 56 kDa Observed Molecular Weight: 56 kDa GenBank Accession Number: BC042141 Gene Symbol: RCC2 Gene ID (NCBI): 55920 RRID: AB_2878311 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P258 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.