Proteintech, 16792-1-AP, PLCD3 Polyclonal antibody

Size: 20ul / 150ul The PLCD3 (16792-1-AP) by Proteintech is a Polyclonal antibody targeting PLCD3 in WB, IHC, ELISA applications with reactivity to human samples 16792-1-AP targets PLCD3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SH-SY5Y cells, A549 cells, U2OS cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions. Specification Tested Reactivity: human Cited Reactivity: human, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10249 Product name: Recombinant human PLCD3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 441-789 aa of BC072384 Sequence: AMARHLCTILGDMLVTQALDSPNPEELPSPEQLKGRVLVKGKKLPAARSEDGRALSDREEEEEDDEEEEEEVEAAAQRRLAKQISPELSALAVYCHATRLRTLHPAPNAPQPCQVSSLSERKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLVNGQCGYVLKPACLRQPDSTFDPEYPGPPRTTLSIQVLTAQQLPKLNAEKPHSIVDPLVRIEIHGVPADCARQETDYVLNNGFNPRWGQTLQFQLRAPELALVRFVVEDYDATSPNDFVGQFTLPLSSLKQGYRHIHLLSKDGASLSPATLFIQIRIQRS Predict reactive species Full Name: phospholipase C, delta 3 Calculated Molecular Weight: 789 aa, 89 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC072384 Gene Symbol: PLCD3 Gene ID (NCBI): 113026 RRID: AB_2878316 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N3E9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.