Proteintech, 16809-1-AP, MAGED4 Polyclonal antibody

Size: 20ul / 150ul The MAGED4 (16809-1-AP) by Proteintech is a Polyclonal antibody targeting MAGED4 in WB, IHC, ELISA applications with reactivity to human samples 16809-1-AP targets MAGED4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, A549 cells Positive IHC detected in: human gliomas tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10329 Product name: Recombinant human MAGED4 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-350 aa of BC081566 Sequence: MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSK Predict reactive species Full Name: melanoma antigen family D, 4 Calculated Molecular Weight: 741 aa, 81 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC081566 Gene Symbol: MAGED4 Gene ID (NCBI): 728239 RRID: AB_2138678 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96JG8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.