Proteintech, 16936-1-AP, RNF10 Polyclonal antibody

Size: 20ul / 150ul The RNF10 (16936-1-AP) by Proteintech is a Polyclonal antibody targeting RNF10 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, Jurkat cells, human brain tissue Positive IP detected in: PC-3 cells Positive IHC detected in: mouse brain tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10433 Product name: Recombinant human RNF10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 463-811 aa of BC016622 Sequence: LPEACDDLELADDNLKEGTICTESSQQEPITKSGFTRLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIAGYSMSEDVRQRHRYLSHLPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEIEENKKQGKYPEVHIPLENLQQFPAFNSYTCSSDSALGPTSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKLDTPATSDPLSEEKGGKKRKKQKQKLLFSTSVVHTK Predict reactive species Full Name: ring finger protein 10 Calculated Molecular Weight: 811 aa, 90 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC016622 Gene Symbol: RNF10 Gene ID (NCBI): 9921 RRID: AB_2181846 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N5U6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.