Proteintech, 16941-1-AP, GFM2 Polyclonal antibody

Size: 20ul / 150ul The GFM2 (16941-1-AP) by Proteintech is a Polyclonal antibody targeting GFM2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 16941-1-AP targets GFM2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue Positive IP detected in: HepG2 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The GFM2 gene encodes a GTPase involved in mitochondrial protein synthesis. GFM2 mediates ribosome recycling but lacks translocation activity, whereas GFM1 catalyzes translocation during elongation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10530 Product name: Recombinant human GFM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 430-779 aa of BC015712 Sequence: GNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVIEFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKALKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLTSGSATFALELSTYQAMNPQDQNTLLNRRSGLT Predict reactive species Full Name: G elongation factor, mitochondrial 2 Calculated Molecular Weight: 779 aa, 87 kDa Observed Molecular Weight: 87 kDa GenBank Accession Number: BC015712 Gene Symbol: GFM2 Gene ID (NCBI): 84340 RRID: AB_2110151 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969S9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.