Proteintech, 16961-1-AP, CA2 Polyclonal antibody

Size: 20ul / 150ul The CA2 (16961-1-AP) by Proteintech is a Polyclonal antibody targeting CA2 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 16961-1-AP targets CA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells Positive IP detected in: mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10680 Product name: Recombinant human CA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC011949 Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK Predict reactive species Full Name: carbonic anhydrase II Calculated Molecular Weight: 260 aa, 29 kDa Observed Molecular Weight: 29 kDa GenBank Accession Number: BC011949 Gene Symbol: CA2 Gene ID (NCBI): 760 RRID: AB_2065857 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00918 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.