Proteintech, 17023-1-AP, COL6A1 Polyclonal antibody

Size: 20ul / 150ul The COL6A1 (17023-1-AP) by Proteintech is a Polyclonal antibody targeting COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 17023-1-AP targets COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, HEK-293T cells, human heart tissue, human skeletal muscle tissue, rat heart tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human colon tissue, human liver tissue, mouse liver tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon tissue, mouse skeletal muscle tissue Positive IF detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF): IF : 1:20-1:200 Background Information Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, zebrafish, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10288 Product name: Recombinant human COL6A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 770-1028 aa of BC052575 Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG Predict reactive species Full Name: collagen, type VI, alpha 1 Calculated Molecular Weight: 1028 aa, 109 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC052575 Gene Symbol: COL6A1 Gene ID (NCBI): 1291 RRID: AB_2229737 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P12109 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.