Proteintech, 17087-1-AP, DDX27 Polyclonal antibody

Size: 20ul / 150ul The DDX27 (17087-1-AP) by Proteintech is a Polyclonal antibody targeting DDX27 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17087-1-AP targets DDX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse liver tissue, NIH/3T3 cells Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9426 Product name: Recombinant human DDX27 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 418-767 aa of BC016060 Sequence: EGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRASPSFEERKQLGLPHQRRGGNFKSKSRYKRRK Predict reactive species Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 Calculated Molecular Weight: 796 aa, 90 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC016060 Gene Symbol: DDX27 Gene ID (NCBI): 55661 RRID: AB_2092569 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96GQ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.