Proteintech, 17097-1-AP, LARS2 Polyclonal antibody

Size: 20ul / 150ul The LARS2 (17097-1-AP) by Proteintech is a Polyclonal antibody targeting LARS2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 17097-1-AP targets LARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family. Specification Tested Reactivity: human Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10576 Product name: Recombinant human LARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 554-903 aa of BC025989 Sequence: VDLYIGGKEHAVMHLFYARFFSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLALVPRKLCAHYTWDASVLLQAWPAVDPEFLQQPEVVQMAVLINNKACGKIPVPQQVARDQDKVHEFVLQSELGVRLLQGRSIKKSFLSPRTALINFLVQD Predict reactive species Full Name: leucyl-tRNA synthetase 2, mitochondrial Calculated Molecular Weight: 903 aa, 102 kDa Observed Molecular Weight: 102 kDa GenBank Accession Number: BC025989 Gene Symbol: LARS2 Gene ID (NCBI): 23395 RRID: AB_2132807 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15031 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.