Proteintech, 17143-1-AP, CKAP2L Polyclonal antibody

Size: 20ul / 150ul The CKAP2L (17143-1-AP) by Proteintech is a Polyclonal antibody targeting CKAP2L in WB, IHC, IP, ELISA applications with reactivity to human samples 17143-1-AP targets CKAP2L in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells, Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human colon cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10909 Product name: Recombinant human CKAP2L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 396-745 aa of BC036217 Sequence: IRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEEEEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLASKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGFQTPES Predict reactive species Full Name: cytoskeleton associated protein 2-like Calculated Molecular Weight: 745 aa, 84 kDa Observed Molecular Weight: 84 kDa GenBank Accession Number: BC036217 Gene Symbol: CKAP2L Gene ID (NCBI): 150468 RRID: AB_2080720 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IYA6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.