Proteintech, 17237-1-AP, FYN Polyclonal antibody

Size: 20ul / 150ul The FYN (17237-1-AP) by Proteintech is a Polyclonal antibody targeting FYN in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17237-1-AP targets FYN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells Positive IHC detected in: rat testis tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family, and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing a greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11080 Product name: Recombinant human FYN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 231-483 aa of BC032496 Sequence: YSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL Predict reactive species Full Name: FYN oncogene related to SRC, FGR, YES Calculated Molecular Weight: 537aa,61 kDa; 482aa,55 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC032496 Gene Symbol: FYN Gene ID (NCBI): 2534 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06241 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.