Proteintech, 17483-1-AP, LUZP1 Polyclonal antibody

Size: 20ul / 150ul The LUZP1 (17483-1-AP) by Proteintech is a Polyclonal antibody targeting LUZP1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 17483-1-AP targets LUZP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human ovary tumor tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information LUZP1, also known as LUZP, is predominantly expressed in the brain. LUZP1 is a centrosome, actin, and midbody-localizing protein implicated in ciliogenesis regulation and actin cytoskeleton stability (PMID: 33869174). LUZP1 null mice present defective neural tube closure and cardiovascular problems, which cause perinatal death. In addition, LUZP1 has also been implicated in the etiology of diseases like the 1p36 and the Townes-Brocks syndromes (PMID: 35738212). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11518 Product name: Recombinant human LUZP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC051733 Sequence: MAEFTSYKETASSRHLRFKLQSLSRRLDELEEATKNLQKAEDELLDLQDKVIQAEGSNSSMLAEIEVLRQRVLRIEGKDEEIKRAEDLCRLMKEKLEEEENLTRELKSEIERLQKRMAELEKLEEAFSRSKNDCTQLCLSLNEERNLTKKISSELEMLRVKVKELESSEDRLDKTEQSLASELEKLKSLTLSFVSERKYLNEKEKENEKLIKELTQKLEQNKKMNRDYTRNASNLERNDLRIEDGISSTLPSKESRRKGGLDYLKQVENETRNKSENEKNRNQEDNKVKDLNQEIEKLKTQIKHFESLEEELKKMKSKNNDLQDNYLSEQNKNKLLASQLEEIKLQIKKQKELENGEV Predict reactive species Full Name: leucine zipper protein 1 Calculated Molecular Weight: 1026 aa, 115 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC051733 Gene Symbol: LUZP1 Gene ID (NCBI): 7798 RRID: AB_2139498 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86V48 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.