Proteintech, 17514-1-AP, Cytokeratin 8 Polyclonal antibody

Size: 20ul / 150ul The Cytokeratin 8 (17514-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 17514-1-AP targets Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells Positive IHC detected in: human appendicitis tissue, human bowen disease tissue, human colon tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, zebrafish, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 8 Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: NM_002273 Gene Symbol: Cytokeratin 8 Gene ID (NCBI): 3856 RRID: AB_2134726 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.