Proteintech, 17594-1-AP, SMYD4 Polyclonal antibody

Size: 20ul / 150ul The SMYD4 (17594-1-AP) by Proteintech is a Polyclonal antibody targeting SMYD4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 17594-1-AP targets SMYD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HepG2 cells Positive IHC detected in: human pancreas tissue, human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development. Specification Tested Reactivity: human, rat Cited Reactivity: human, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11552 Product name: Recombinant human SMYD4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 201-578 aa of BC035077 Sequence: KDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLVQEDAFVSVLNPGELPPPHHGLDSKWDTRVTNGDLYCHRCLKHTLATVPCDGCSYAKYCSQECLQQVWELYHRTECPLGGLLLTLGVFCHIALRLTLLVGFEDVRKIITKLCDKISNKDICLPESNNQVKTLNYGLGESEKNGNIVETPIPGCDINGKYENNYNAVFNLLPHTENHSPEHKFLCALCVSALCRQLEAASLQAIPTERIVNSSQLKAAVTPELCPDVTIWGVAMLRHMLQLQCNAQAMTTIQHTGPKGSIVTDSRQVRLATGIFPVISLLNHSCSPNTSVSFISTVATIRASQRIRKGQEILHCYGPHKS Predict reactive species Full Name: SET and MYND domain containing 4 Calculated Molecular Weight: 804 aa, 89 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC035077 Gene Symbol: SMYD4 Gene ID (NCBI): 114826 RRID: AB_10694429 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IYR2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.