Proteintech, 17687-1-AP, PJA1 Polyclonal antibody

Size: 20ul / 150ul The PJA1 (17687-1-AP) by Proteintech is a Polyclonal antibody targeting PJA1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 17687-1-AP targets PJA1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells Positive IP detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11820 Product name: Recombinant human PJA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 294-643 aa of BC075803 Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL Predict reactive species Full Name: praja ring finger 1 Calculated Molecular Weight: 643 aa, 71 kDa Observed Molecular Weight: 71-80 kDa GenBank Accession Number: BC075803 Gene Symbol: PJA1 Gene ID (NCBI): 64219 RRID: AB_2252483 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NG27 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.