Proteintech, 17722-1-AP, SMARCC1 Polyclonal antibody

Size: 20ul / 150ul The SMARCC1 (17722-1-AP) by Proteintech is a Polyclonal antibody targeting SMARCC1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 17722-1-AP targets SMARCC1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, Jurkat cells, rat brain tissue, rat testis tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human malignant melanoma tissue, human appendicitis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12109 Product name: Recombinant human SMARCC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 756-1105 aa of BC050564 Sequence: GLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENETDEGDKAQDGENEKNSEKEQDSEVSEDTKSEEKETEENKELTDTCKERESDTGKKKVEHEISEGNVATAAAAALASAATKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREKEALEQQRQQLLTERQNFHMEQLKYAELRARQQMEQQQHGQNPQQAHQHSGGPGLAPLGAAGHPGMMPHQQPPPYPLMHHQMPPPHPPQPGQIPGPGSMMPGQHMPGRMIPTVAANIHPSGSGPTPPGMPPMPGNILGPRVPLTAPNGMYPPPPQQQPPPPPPADGVPPPPAPGPPASAAP Predict reactive species Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 Calculated Molecular Weight: 1105 aa, 123 kDa Observed Molecular Weight: 155-160 kDa GenBank Accession Number: BC050564 Gene Symbol: SMARCC1 Gene ID (NCBI): 6599 RRID: AB_2191987 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92922 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.