Proteintech, 17771-1-AP, PDE7B Polyclonal antibody

Size: 20ul / 150ul The PDE7B (17771-1-AP) by Proteintech is a Polyclonal antibody targeting PDE7B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17771-1-AP targets PDE7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PDE7B(cAMP-specific 3',5'-cyclic phosphodiesterase 7B) belongs to the cyclic nucleotide phosphodiesterase family and PDE7 subfamily,which regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B gene is expressed predominantly in the brain and in a number of other tissues including liver, heart, thyroid glands, and skeletal muscle, but not expressed in blood leukocytes (PMID:12388353). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12184 Product name: Recombinant human PDE7B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-450 aa of BC075082 Sequence: MSCLMVERCGEILFENPDQNAKCVCMLGDIRLRGQTGVRAERRGSYPFIDFRLLNSTTYSGEIGTKKKVKRLLSFQRYFHASRLLRGIIPQAPLHLLDEDYLGQARHMLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFNTHGLIHHFKLDMVTLHRFLVMVQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGLLAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRESRLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEDAQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGELEQKFELEISPLCNQQKDSIPSIQIGFMSYIVEPLFREWAHFTGNSTLSENMLGHLAHNKAQWKSLLPRQHRSRGSSGSGPDHDHAGQGTESEEQEGDSP Predict reactive species Full Name: phosphodiesterase 7B Calculated Molecular Weight: 450 aa, 52 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC075082 Gene Symbol: PDE7B Gene ID (NCBI): 27115 RRID: AB_2268032 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NP56 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.