Proteintech, 17813-1-AP, SPRY1 Polyclonal antibody

Size: 20ul / 150ul The SPRY1 (17813-1-AP) by Proteintech is a Polyclonal antibody targeting SPRY1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 17813-1-AP targets SPRY1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A2780 cells, HEK-293 cells, HepG2 cells Positive IP detected in: HT-29 cells, A2780 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Sprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras/MEK/ERK pathway (PMID: 21310958). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12129 Product name: Recombinant human SPRY1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-319 aa of BC063856 Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS Predict reactive species Full Name: sprouty homolog 1, antagonist of FGF signaling (Drosophila) Calculated Molecular Weight: 319 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC063856 Gene Symbol: SPRY1 Gene ID (NCBI): 10252 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43609 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.