Proteintech, 17818-1-AP, SNCAIP Polyclonal antibody

Size: 20ul / 150ul The SNCAIP (17818-1-AP) by Proteintech is a Polyclonal antibody targeting SNCAIP in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 17818-1-AP targets SNCAIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, fetal human brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12147 Product name: Recombinant human SNCAIP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 253-603 aa of BC094759 Sequence: ILRQLLGKEISENVCTQEKLSLEFQDAQASSRNSKKIPLEKRELKLARLRQLMQRSLSESDTDSNNSEDPKTTPVRKADRPRPQPIVESVESMGSAESLHLMIKKHTLASGGRRFPFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIPPNQPSGDPQQPSPDSTAAQKVATSPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDKGRTLQRTSTSNESGDQLKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASSASKGKNKAEMYSSCINLSSNMLIEEHLCNDTRHNDINRKMKKSYSIKHIAEPESKELFL Predict reactive species Full Name: synuclein, alpha interacting protein Calculated Molecular Weight: 603aa,67 kDa; 1016aa,111 kDa Observed Molecular Weight: 100 kDa, 75 kDa GenBank Accession Number: BC094759 Gene Symbol: SNCAIP Gene ID (NCBI): 9627 RRID: AB_2878444 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6H5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.