Proteintech, 17950-1-AP, GPR177 Polyclonal antibody

Size: 20ul / 150ul The GPR177 (17950-1-AP) by Proteintech is a Polyclonal antibody targeting GPR177 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 17950-1-AP targets GPR177 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U-87 MG cells, mouse brain tissue, HEK-293 cells, rat brain tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human ovary tissue, mouse liver tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12428 Product name: Recombinant human GPR177 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 34-239 aa of BC110826 Sequence: LIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKT Predict reactive species Full Name: G protein-coupled receptor 177 Calculated Molecular Weight: 541 aa, 62 kDa Observed Molecular Weight: 46-65 kDa GenBank Accession Number: BC110826 Gene Symbol: GPR177 Gene ID (NCBI): 79971 RRID: AB_2034928 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5T9L3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.