Proteintech, 18098-1-AP, B3GNT3 Polyclonal antibody

Size: 20ul / 150ul The B3GNT3 (18098-1-AP) by Proteintech is a Polyclonal antibody targeting B3GNT3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 18098-1-AP targets B3GNT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse lung tissue, SH-SY5Y cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) is a type II transmembrane protein located in the Golgi apparatus and contributes to the regulation of L-selectin ligand function, lymphocyte trafficking, and T cell homing. B3GNT3 is overexpressed in breast cancer, and also plays a critical role in the aggressiveness of pancreatic cancer (PMID: 33718036, 30466404). Western blot analysis detected B3GNT3 at an apparent molecular mass of 43 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12061 Product name: Recombinant human B3GNT3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 34-372 aa of BC066876 Sequence: CKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHYRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQHLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY Predict reactive species Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 Calculated Molecular Weight: 372 aa, 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC066876 Gene Symbol: B3GNT3 Gene ID (NCBI): 10331 RRID: AB_10596928 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2A9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.