Proteintech, 18178-1-AP, RBM24 Polyclonal antibody

Size: 20ul / 150ul The RBM24 (18178-1-AP) by Proteintech is a Polyclonal antibody targeting RBM24 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 18178-1-AP targets RBM24 in WB, IHC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12871 Product name: Recombinant human RBM24 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC104808 Sequence: MYVCLCVSVAKVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRIMQPGFAFGVQQLHPALIQRPFGIPAHYVYPQAFVQPGVVIPHVQPTAAAASTTPYIDYTGAAYAQYSAAAAAAAAAAAYDQYPYAASPAAAGYVTAGGYGYAVQQPITAAAPGTAAAAAAAAAAAAAFGQYQPQQLQTDRMQ Predict reactive species Full Name: RNA binding motif protein 24 Calculated Molecular Weight: 191 aa, 20 kDa Observed Molecular Weight: 18-25 kDa GenBank Accession Number: BC104808 Gene Symbol: RBM24 Gene ID (NCBI): 221662 RRID: AB_2878513 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BX46 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.