Proteintech, 18304-1-AP, Thrombospondin 1 Polyclonal antibody

Size: 20ul / 150ul The Thrombospondin 1 (18304-1-AP) by Proteintech is a Polyclonal antibody targeting Thrombospondin 1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human peripheral blood platelets, A431 cells, C6 cells, HeLa cells, HUVEC cells, NIH/3T3 cells, RAW 264.7 cells Positive IHC detected in: human lung cancer tissue, human liver tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: thrombospondin 1 Calculated Molecular Weight: 129 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC028145 Gene Symbol: Thrombospondin 1 Gene ID (NCBI): 7057 RRID: AB_2201959 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07996 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.