Proteintech, 18321-1-AP, FBXW5 Polyclonal antibody

Size: 20ul / 150ul The FBXW5 (18321-1-AP) by Proteintech is a Polyclonal antibody targeting FBXW5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 18321-1-AP targets FBXW5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, K-562 cells Positive IHC detected in: mouse testis tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13163 Product name: Recombinant human FBXW5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 217-566 aa of BC014297 Sequence: VLWLNNAFQDVESENVNVVKRLFKIQNLNASTVRTVMVADCSRFDSPDLLLEAGDPATSPCRIFDLGSDNEEVVAGPAPAHAKEGLRHFLDRVLEGRAQPQLSERMLETKVAELLAQGHTKPPERSATGAKSKYLIFTTGCLTYSPHQIGIKQILPHQMTTAGPVLGEGRGSDAFFDALDHVIDIHGHIIGMGLSPDNRYLYVNSRAWPNGAVVADPMQPPPIAEEIDLLVFDLKTMREVRRALRAHRAYTPNDECFFIFLDVSRDFVASGAEDRHGYIWDRHYNICLARLRHEDVVNSVVFSPQEQELLLTASDDATIKAWRSPRTMRVLQAPRPRPRTFFSWLASQRR Predict reactive species Full Name: F-box and WD repeat domain containing 5 Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC014297 Gene Symbol: FBXW5 Gene ID (NCBI): 54461 RRID: AB_2878530 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969U6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.